Retry the protocol with freshly made dialysis buffer, remaining very watchful to incorporate the proper amount of NaCl The subsequent posting describes a variety of disruption procedures, and indicates which approach to utilize for certain tissues/cell varieties. Every one of the disruption procedures explained here are compatible with Ambion's RNA https://www.quora.com/profile/Even-Sa/What-are-the-application-areas-of-nucleic-acid-extraction-reagents-Recommended-application-areas-Enzyme-digestion-o